Antioxidant and Anti-inflammatory Studies of Aqueous and Methanol Extracts of Pterocarpus erinaceus poir Leaves
DOI:
https://doi.org/10.26538/tjdr/v2i4.4Keywords:
Polyphenol, Anti-inflammatory, Metabolite, Erythrocytes haemolysis, Metabolic syndromeAbstract
Purpose: Increasing ethno-medicinal adoption of plant based management option for chronic diseases is well documented. Phytochemistry studies have shown presence of metabolites in plants with diverse pharmacological actions. This study evaluates antioxidant and anti-inflammatory potentials in Pterocarpus erinaceus leaf extracts.
Method: The 2, 2-diphenyl-1-picryl-hydrazyl (DPPH) radical reduction and ferric-reducing antioxidant power (FRAP) were assayed for antioxidant evaluation. Human red blood cell membrane stabilization and the egg albumin (protein) denaturation inhibition were determined for the anti-inflammatory.
Results: Significant concentrations of phenols, flavonoids, tannins, saponins, vitamins A, C and E were detected in the extract. The extract demonstrated a high total antioxidant capacity (TAC), DPPH radical was inhibited, IC50 0.70 μg/ ml (methanol) and 3.65 μg/ ml (aqueous), ferric pyridyltriazine (Fe3+-TPTZ) complex was also reduced to the ferrous ion (Fe2+) complex. Additionally, both extract inhibited protein denaturation and erythrocyte haemolysis. Aqueous extract however, produced more potency on protein, possessing a higher % protection with a lower IC50 of 0.24 μg/ ml, not significantly different from aspirin.
Conclusion: Aqueous and methanol leaf extracts of P. erinaceus in this study demonstrated antioxidant and anti-inflammatory potentials. Vitamins and polyphenols in the extracts might have enhance the antioxidant and anti-inflammatory activities, therefore possible therapeutic effect of the plant could include antioxidant and anti-inflammation mechanisms.
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