Phytochemical investigation and quantification of bioactive compounds of Rhamnus alaternus (L.)
DOI:
https://doi.org/10.26538/tjdr/v1i1.3Keywords:
organic extracts, aqueous extracts, bioactive compounds, assay, screening, phytochemical study, Rhamnus alaternusAbstract
Purpose: In the context of the valorization of medicinal plants in the region of Taza-Morocco, the present study is focused on a phytochemical study of the Rhamnus alaternus plant in view of its use in traditional medicine and its beneficial health properties.
Methods: The phytochemical research is carried out through aqueous and organic extraction processes in two modalities (hot and cold), characterization and quantification of its bioactive compounds.
Results: The results of the phytochemical study revealed that the decocted and methanolic extract had the highest yields, with percentages of around 42.9% and 37.5% respectively. The leaves of this plant are rich in flavonoids, tannins, saponins, sterols, anthracenosides and quinones. The decocted had the highest averages for phenolic compounds among the aqueous extracts, with values of 21.67 ± 0.10 μg GAE/mg E for polyphenols, 58.40 ± 0.33 μg QE/mg E for flavonoids and 5.43 ± 0.01 μg CE/mg E for tannins. While for organic extracts, the highest concentrations of polyphenols were obtained by ethyl acetate extract with 59.23 ± 0.72 μg GAE/mg E, those of flavonoids and tannins by chloroformic extract with respectively: 470. 79 ± 1.70 μg QE/mg E and 51.75 ± 0.14 μg CE/mg E.
Conclusion: Rhamnus alaternus is an attractive plant to study, given its wealth of bioactive compounds involved in interesting biological activities.
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Alaoui MSB, Satrani B, Boussoula E, Ghanmi M. Ethnobotanical study of medicinal plants used in the Moroccan Sahara provinces. Int J Innov Appl Stud. 2018;24(2):789-801.
Kahouadji MS, Hseini S, Jaafar B, Zaid Y, Kahouadji A, Zidane L. Floristic diversity and evaluation of the potential of spontaneous medicinal plants in the Bigoudine Watershed (Moroccan Western High Atlas). Int J For Res. 2022;2022(1):2485502. https://doi.org/10.1155/2022/2485502
Jamaleddine M, El Oualidi J, Taleb MS, Thévenin T, El Alaoui-Faris FE Inventory and conservation status of aromatic and medicinal plants in Morocco. Phytothérapie. 2017;15(3):114122. https://doi.org/10.1007/s10298-017-1131-2
Menioui M. Biological diversity in Morocco. In: Global Biodiversity. Apple Academic Press; 2018. p. 133-171
El Alami A, Chait A. Ethnopharmacological and ethnobotanical survey of medicinal plants in the central High Atlas of Morocco. Alger J Nat Prod. 2017;5(1):427-445. https://doi.org/10.5281/zenodo.843894
Redouan FZ, Benítez G, Picone RM, Crisafulli A, Yebouk C, Bouhbal M, Ben Driss A, Kadiri M, Molero-Mesa J, Merzouki A. Traditional medicinal knowledge of Apiaceae at Talassemtane National Park (northern Morocco). S Afr J Bot. 2020;131:118-130. https://doi.org/10.1016/j.sajb.2020.02.004
Shakya AK. Medicinal plants: Future source of new drugs. Int J Herb Med. 2016;4(4):59-64.
Boukhatem MN, Setzer WN. Aromatic herbs, medicinal plant-derived essential oils, and phytochemical extracts as potential therapies for coronaviruses: future perspectives. Plants. 2020;9(6):800. https://doi.org/10.3390/plants9060800
Boontha S, Thoedyotin T, Saengtabtim T, Im-erb P, Chaniad N, Buranrat B, Pitaksuteepong T. Cytotoxic, colony formation and anti-migratory effects of Spilanthes acmella (Asteraceae) aerial extract on MCF-7 cells and its cream formulation. Trop J Pharm Res. 2020;19(1):17-24. http://dx.doi.org/10.4314/tjpr.v19i1.3
Alshahrani SM. SWOT analysis of herbal medicine in Saudi Arabia: Current practices and prospects. Trop J Pharm Res. 2024;23(11):1933-1938. http://dx.doi.org/10.4314/tjpr.v23i11.18
Bachar M, Zidane L, Rochdi A. Ethno-medicinal and traditional phytotherapy of plants used in Bouhachem Natural Regional Park “Rif of Morocco” - case of Tazroute district. J Mater Environ Sci. 2016;7(11):4175-4204.
Boulfia M, Lamchouri F, Khabbach A, Zalaghi A, Assem N, Toufik H. An ethnopharmacological evaluation of Moroccan medicinal plants of the middle atlas and pre-rif of the province of Taza. J Chem Pharm Res. 2018;10(7):156-173.
Salhi N, Bouyahya A, Fettach S, Zellou A, Cherrah Y. Ethnopharmacological study of medicinal plants used in the treatment of skin burns in occidental Morocco (area of Rabat). S Afr J Bot. 2019;121:128-42. https://doi.org/10.1016/j.sajb.2018.10.038
Boulfia M, Lamchouri F, Lachkar N, Khabbach A, Zalaghi A, Toufik H. Socio-economic value and ethnobotanical study of Moroccan wild Leopoldia comosa L. Parl. bulbs. Ethnobot Res Appl. 2021;21:1-17. http://dx.doi.org/10.32859/era.21.05.1-17
Lachkar N, Lamchouri F, Toufik H. Ethnopharmacological Survey, Mineral and Chemical Content, In Vitro Antioxidant, and Antibacterial Activities of Aqueous and Organic Extracts of Chamaerops humilis L. var. argentea Andre Leaves. BioMed Res Int. 2022;2022:127. https://doi.org/10.1155/2022/1091247
Mahtal A, Lamchouri F, Toufik H. Morchella esculenta (L.) Pers. Wild of the Province of Taza, Morocco: Ethnomedicinal and socio-economic survey and perspectives. Trop J Nat Prod Res. 2024;8(11):9019-9026.https://doi.org/10.26538/tjnpr/v8i11.9
Eddouks M, Ouahidi ML, Farid O, Moufid A, Khalidi A, Lemhadri A. The use of medicinal plants in the treatment of diabetes in Morocco. Phytothérapie. 2007;5(4):194203. https://doi.org/10.1007/s10298-007-0252-4
Chaachouay N, Benkhnigue O, Khamar H, Zidane L. Ethnobotanical study of medicinal and aromatic plants used in the treatment of genito-urinary diseases in the Moroccan Rif. J Mater Environ Sci. 2020;11(1):15-29.
Hakkou S, Sabir M, Machouri N. Contribution to the study of the production factors of Moroccan truffles (Soil, Climate, Vegetation). J Mater Environ Sci. 2023;14(1):13-30.
Chellik S, Dbiba Y, Mansouri I, Squalli W, Achiban H, El Agy A, Zidane L. Characterization of Natural Ecosystems, Biodiversity, Socioeconomic and Infrastructures for Sustainable Tourism in the National Park of Tazekka in Taza Province (Morocco). Ecol Eng Environ Technol. 2024;25(5):167178. https://doi.org/10.12912/27197050/184225
Harroussi BE, Chakor A, Belmaati H. Territorial marketing: what approach to marketing the brand image and strengthening the positioning and dynamics of ecotouristic destinations “case of Tazekka National Park”. Rev Int Mark Manag Strateg. 2019;1(4).
Gulías J, Traveset A, Riera N, Mus M. Critical stages in the recruitment process of Rhamnus alaternus L. Ann Bot. 2004;93(6):723-731.
Ben Ammar R, Kilani S, Bouhlel I, Skandrani I, Naffeti A, Boubaker J, Ben Sghaier M, Bhouri W, Mahmoud A, Chekir-Ghedira L, Ghedira K.
Antibacterial and cytotoxic activities of extracts from (Tunisian) Rhamnus alaternus (Rhamnaceae). Ann Microbiol. 2007;57(3):453. https://doi.org/10.1007/BF03175089
Benchiha W, Mahroug S, Aoued L, Bouterfas K Antihepatoxic activity of Rhamnus alaternus L. (Rhamnaceae) leaves extracts. Phytothérapie. 2017;15(1):10. https://doi.org/10.1007/s10298-015-1012-5
Moussi K, Nayak B, Perkins LB, Dahmoune F, Madani K, Chibane M. HPLC-DAD profile of phenolic compounds and antioxidant activity of leaves extract of Rhamnus alaternus L. Ind Crops Prod. 2015;74:858866. https://doi.org/10.1016/j.indcrop.2015.06.015
Longo L, Vasapollo G, Rescio L. Identification of Anthocyanins in Rhamnus alaternus L. Berries. J Agric Food Chem. 2005;53(5):17231727. https://doi.org/10.1021/jf048253p
Kosalec I, Kremer D, Locatelli M, Epifano F, Genovese S, Carlucci G, Randić M, Zovko Končić M. Anthraquinone profile, antioxidant and antimicrobial activity of bark extracts of Rhamnus alaternus, R. fallax, R. intermedia and R. pumila. Food Chem. 2013;136(2):335341. https://doi.org/10.1016/j.foodchem.2012.08.026
Bhouri W, Ben Sghaier M, Kilani S, Bouhlel I, Dijoux-Franca MG, Ghedira K, Chekir Ghedira L. Evaluation of antioxidant and antigenotoxic activity of two flavonoids from Rhamnus alaternus L. (Rhamnaceae): Kaempferol 3-O-β-isorhamninoside and rhamnocitrin 3-O-β-isorhamninoside. Food Chem Toxicol. 2011;49(5):11671173. https://doi.org/10.1016/j.fct.2011.02.011
Zeouk I, Ouedrhiri W, Jiménez IA, Lorenzo-Morales J, Bazzocchi IL, Bekhti K. Intra-combined antioxidant activity and chemical characterization of three fractions from Rhamnus alaternus extract: Mixture design. Ind Crops Prod. 2020;144:112054. https://doi.org/10.1016/j.indcrop.2019.112054
Tacherfiout M, Petrov PD, Mattonai M, Ribechini E, Ribot J, Bonet ML, Khettal H. Antihyperlipidemic effect of a Rhamnus alaternus leaf extract in Triton-induced hyperlipidemic rats and human HepG2 cells. Biomed Pharmacother. 2018;101:501-509. https://doi.org/10.1016/j.biopha.2018.02.106
Gadouche L, Zerrouki K, Zidane A, Ababou A, Bachir Elazaar I, Merabet D, Henniche W, Ikhlef S. Genoprotective, antimutagenic, and antioxidant effects of methanolic leaf extract of Rhamnus alaternus L. from the Bissa mountains in Algeria. Foods Raw Mater. 2022;196205.
Amtaghri S, Farid O, Lahrach N, Slaoui M, Eddouks M. Antihyperglycemic effect of Rhamnus alaternus L. aqueous extract in streptozotocin-induced diabetic rats. Cardiovasc Hematol Disord Drug Targets. 2022;22(4):245-255. https://doi.org/10.2174/1871529X23666230123123317
Bouabid K, Lamchouri F, Toufik H, Sayah K, Cherrah Y, Faouzi MEA. Phytochemical screening and in vitro evaluation of alpha amylase, alpha glucosidase and beta galactosidase inhibition by aqueous and organic Atractylis gummifera L. extracts. Plant Sci Today. 2018;5(3):103112. https://doi.org/10.14719/pst.2018.5.3.393
Bouabid K, Lamchouri F, Toufik H, Faouzi MEA. Phytochemical investigation, in vitro and in vivo antioxidant properties of aqueous and organic extracts of toxic plant: Atractylis gummifera L. J Ethnopharmacol. 2020;253:112640. https://doi.org/10.1016/j.jep.2020.112640
Boulfia M, Lamchouri F, Toufik H. Chemical Analysis, Phenolic Content, and Antioxidant Activities of Aqueous and Organic Moroccan Juglans regia L. Bark Extracts. Curr Bioact Compd. 2020;16(9):13281339. https://doi.org/10.2174/1573407216666200203103354
Senhaji S, Lamchouri F, Bouabid K, Assem N, El Haouari M, Bargach K, Toufik H. Phenolic Contents and Antioxidant Properties of Aqueous and Organic Extracts of a Moroccan Ajuga iva Subsp. Pseudoiva. J Herbs Spices Med Plants. 2020;26(3):248266. https://doi.org/10.1080/10496475.2019.1709249
Senhaji S, Lamchouri F, Toufik H. Phytochemical content, antibacterial and antioxidant potential of endemic plant Anabasis aretioïdes Coss. & Moq. (Chenopodiaceae). Biomed Res Int. 2020;2020. https://doi.org/10.1155/2020/6152932
Lachkar N, Lamchouri F, Bouabid K, Boulfia M, Senhaji S, Stitou M, Toufik H. Mineral Composition, Phenolic Content, and In Vitro Antidiabetic and Antioxidant Properties of Aqueous and Organic Extracts of Haloxylon scoparium Aerial Parts. Evid Based Complement Alternat Med. 2021;2021: 9011168. https://doi.org/10.1155/2021/9011168
Lachkar N, Lamchouri F, Bouabid K, Senhaji S, Boulfia M, Akabli T, Zalaghi A, Toufik H. In vitro study of the antimitotic power and in vivo acute toxicity of aqueous and organic extracts of the aerial part of Haloxylon scoparium Pomel. and evaluation of the correlation between the chemical profile and their biological activities. Plant Sci Today. 2023; 10(1):242-251. https://doi.org/10.14719/pst.2001
Mahtal A, Lamchouri F, Toufik H. Mineral composition, phenolic content and antibacterial activity of aqueous and organic extracts of wild Morchella esculenta (L.) Pers. from the province of Taza, Morocco. Biochem Res Int. 2024;2024(1):6769243. https://doi.org/10.1155/bri/6769243
Boulfia M, Lamchouri F, Senhaji S, Lachkar N, Bouabid K, Toufik H. Mineral Content, Chemical Analysis, In Vitro Antidiabetic and Antioxidant Activities, and Antibacterial Power of Aqueous and Organic Extracts of Moroccan Leopoldia comosa (L.) Parl. Bulbs. Evid Based Complement Alternat Med. 2021;2021: :9932291. https://doi.org/10.1155/2021/9932291
Herzi N, Bouajila J, Camy S, Romdhane M, Condoret JS. Comparison of different methods for extraction from Tetraclinis articulata: Yield, chemical composition and antioxidant activity. Food Chem. 2013;141(4):35373545. https://doi.org/10.1016/j.foodchem.2013.06.065
Boussahel S, Dahamna S, Ruberto G, Siracusa L, Harzallah D. Phytochemical Study and Antioxidant Activities of Leaves Extracts from Rhamnus alaternus L. Pharmacogn Commun. 2013;3(1):4653. https://doi.org/10.5530/pc.2013.1.10
Najjaa H, Neffati M, Zouari S, Ammar E. Essential oil composition and antibacterial activity of different extracts of Allium roseum L., a North African endemic species. Comptes Rendus Chim. 2007;10(9):820826. https://doi.org/10.1016/j.crci.2007.03.003
Senhaji S, Lamchouri F, Boulfia M, Lachkar N, Bouabid K, Toufik H. Mineral composition, content of phenolic compounds and in vitro antioxidant and antibacterial activities of aqueous and organic extracts of the seeds of Peganum harmala L. South Afr J Bot. 2022;147:697712. https://doi.org/10.1016/j.sajb.2022.03.005
Bouchenak O, Yahiaoui K, Benhabyles N, Laoufi R, Toubal S, El Haddad D, Oussaid S, Blizak D, Arab K. Phytochemical screening and evaluation of the antioxidant power of leaves of Myrtus communis L. and Rhamnus Alaternus L. Rev Agrobiologia. 2020;10(1):1749-1761.
Ben Ammar R, Kilani S, Bouhlel I, Ezzi L, Skandrani I, Boubaker J, Ben Sghaier M, Naffeti A, Mahmoud A, Chekir-Ghedira L, Ghedira K.. Antiproliferative, Antioxidant, and Antimutagenic Activities of Flavonoid-Enriched Extracts from (Tunisian) Rhamnus alaternus L.: Combination with the Phytochemical Composition. Drug Chem Toxicol. 2008;31(1):6180. https://doi.org/10.1080/01480540701688725
Ben Ammar R, Bhouri W, Ben Sghaier M, Boubaker J, Skandrani I, Neffati A, Bouhlel I, Kilani S, Mariotte AM, Chekir-Ghedira L, Dijoux-Franca MG, Ghedira K. Antioxidant and free radical-scavenging properties of three flavonoids isolated from the leaves of Rhamnus alaternus L. (Rhamnaceae) : A structure-activity relationship study. Food Chem. 2009;116(1):258264. https://doi.org/10.1016/j.foodchem.2009.02.043
Mezouar D, Aissaoui M, Benmessaoud A, Lahfa FB. Phytochemical analysis and alpha-amylase inhibitory property of olive (Olea europaea L.) leaves extracts. J Nat Prod Res Appl. 2022;1(3):56-67. https://doi.org/10.46325/jnpra.v1i03.28
Yahyaoui M, Ghazouani N, Sifaoui I, Abderrabba M. Comparison of the Effect of Various Extraction Methods on the Phytochemical Composition and Antioxidant Activity of Thymelaea hirsuta L. aerial parts in Tunisia. Biosci Biotechnol Res Asia. 2017;14(3):9971007. http://dx.doi.org/10.13005/bbra/2534
Yahyaoui M, Bouajila J, Cazaux S, Abderrabba M. The impact of regional locality on chemical composition, anti-oxidant and biological activities of Thymelaea hirsuta L. extracts. Phytomedicine. 2018;41:1323. https://doi.org/10.1016/j.phymed.2018.01.010
Borges CV, Minatel IO, Gomez-Gomez HA, Lima GPP. Medicinal plants: Influence of environmental factors on the content of secondary metabolites. In: Medicinal plants and environmental challenges. 2017. p. 259-277. https://doi.org/10.1007/978-3-319-68717-9_15
Yang L, Wen KS, Ruan X, Zhao YX, Wei F, Wang Q. Response of plant secondary metabolites to environmental factors. Molecules. 2018;23(4):762. https://doi.org/10.3390/molecules23040762
Li Y, Kong D, Fu Y, Sussman MR, Wu H. The effect of developmental and environmental factors on secondary metabolites in medicinal plants. Plant Physiol Biochem. 2020;148:80-89. https://doi.org/10.1016/j.plaphy.2020.01.006
Kabtni S, Sdouga D, Bettaib Rebey I, Save M, Trifi-Farah N, Fauconnier ML, Marghali S. Influence of climate variation on phenolic composition and antioxidant capacity of Medicago minima populations. Sci Rep. 2020;10(1):8293. https://doi.org/10.1038/s41598-020-65160-4
Wang J, Liu K, Li X, Bi K, Zhang Y, Huang J, Zhang R. Variation of active constituents and antioxidant activity in Scabiosa tschiliensis Grunning from different stages. J Food Sci Technol. 2017;54(8):22882295. https://doi.org/10.1007/s13197-017-2666-9
Piao S, Liu Q, Chen A, Janssens IA, Fu Y, Dai J, Liu L, Lian X, Shen M, Zhu X. Plant phenology and global climate change: Current progresses and challenges. Global Change Biol. 2019;25(6):1922-1940. https://doi.org/10.1111/gcb.14619
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