Effects of 2’-hydroxychalcones against Oxidative Stress and Lipid Peroxidation Biomarkers in Paclitaxel-Induced Peripheral Neuropathy in Drosophila Melanogaster (Harwich Strain)
DOI:
https://doi.org/10.26538/tjdr/v2i5.3Keywords:
Chemotherapy-induced peripheral neuropathy (CIPN), Paclitaxel, 2’-hydroxychalcones, Superoxide Dismutase (SOD) Reduced Glutathione (GSH),, Malondialdehyde (MDA), Drosophila melanogasterAbstract
Purpose: Chemotherapy-induced peripheral neuropathy (CIPN), a common adverse effect of paclitaxel treatment, significantly impacts patients' quality of life. This study investigates the potential of 2’-hydroxychalcones, natural compounds with neuroprotective properties, to alleviate paclitaxel-induced peripheral neuropathy (PIPN).
Methods: Leveraging the Drosophila melanogaster model, the research evaluates the compounds' effects on oxidative stress markers, Superoxide Dismutase (SOD), Reduced Glutathione (GSH) and lipid peroxidation biomarker Malondialdehyde (MDA). Flies were grouped into five groups (groups 1 to 5). Group 1 served as a positive control, group 2 the negative control exposed to Paclitaxel only, while groups 3 and 4 were served 100mg and 150mg/10ml feed 2’-hydroxychalcones, respectively, and group 5 was served Gabapentin, the standard drug. The flies were later on homogenized and the homogenates were used to assay for oxidative stress biomarkers (SOD, GSH) and lipid peroxidation (MDA) by spectrometry. Our results reveal that Paclitaxel exposure leads to significant impairments in ROS. The administration of 2’-hydroxychalcones (100mg and 150mg) mitigated this oxidative damage.
Results: These results underscore the potential of chalcones as alternative therapeutic agents for CIPN, offering a foundation for further preclinical and clinical investigations.
Conclusion: By bridging the gap between natural product pharmacology and neuropathy research, the study provides insights into novel strategies for managing CIPN.
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