Histological Investigation of the Protective Effect of Lonchocarpus griffonianus (Baill.) Dunn (Fabaceae) Stem Bark Extract on Aluminium Chloride-induced Testicular Toxicity in Albino Mice
DOI:
https://doi.org/10.26538/tjdr/v2i9.2Keywords:
Lonchocarpus griffonianus, testicular toxicity, Aluminium chloride, Albino mice, methanol extractAbstract
Purpose: Complications associated with male infertility, such as relationship issues and stress brought on by not being able to conceive, as well as the cost of fertility treatments, have led to a desire for safe, inexpensive, and easily accessible fertility-enhancing herbal remedies. Lonchocarpus griffonianus (Fabaceae) is utilized by the natives of Akwa Ibom State, Nigeria, to treat pharyngopulmonary disorders, stomachaches, infertility, amenorrhea, and inflammatory disorders. This study aims to validate the use of L. griffonianus in the management of infertility in ethnomedicine.
Methods: L. griffonianus stem bark was collected, identified, dried, pulverised, and then extracted with absolute methanol. Aluminium chloride (4.3mg/kg) was given intraperitoneally to the animals to induce reproductive toxicity for 28 days. The extract was administered at graded doses of 100, 200, and 400 mg/kg orally, along with Aluminium chloride, for 28 days. On day 29, the animals were sacrificed under anaesthesia. The animal’s testes were harvested for histological analysis.
Results: The demonstrated significant restorative effects on aluminium chloride–induced testicular toxicity in male mice. The standard control group exhibited normal testicular histoarchitecture with intact germ cells and spermatozoa. In contrast, the disease control group showed vacuolated connective tissue, atrophied spermatids, and scanty spermatozoa. Treatment with the extract (100–400 mg/kg) markedly improved spermatogenesis.
Conclusion: L. griffonianus stem bark extract attenuated AlCl3-induced testicular toxicity, indicating its potential as a lead for developing herbal therapies against male infertility.
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