In vivo analysis of Neuromodulatory and Biochemical Effects of Lacatomtom Drinks on Wistar Rat Brain
DOI:
https://doi.org/10.26538/tjdr/v2i5.1Keywords:
Lacatomtom, Neurotoxicity, ATPase, Acetylcholinesterase, butyrylcholinesterase, lacasera, PsychoactiveAbstract
Purpose: This study investigated the neuromodulatory and biochemical effects of Lacatomtom, a mixture of a soft drink and candy, on Wistar rat brains.
Methods: This research examined the activities of ATPases (Mg2+, Na+/K+, Ca2+/Mg2+), acetylcholinesterase (AChE), butyrylcholinesterase (BChE), and levels of dopamine, serotonin, tumor necrosis factor-alpha (TNF-α), sodium ions (Na+), glutathione peroxidase (GPx), bicarbonate (HCO3-), and creatinine kinase (CK). Wistar rats were divided into four groups: control (water), Lacatomtom (LTT), Lacasera (LC) alone, and Tomtom candy dissolved in water (TTW).
Results: Results revealed significant increases in AChE and BChE activities, particularly in the LTT group, suggesting potential cholinergic dysfunction. Elevated ATPase activity was observed in the LTT and LC groups, indicating altered energy metabolism. Furthermore, increased levels of nitrite and protein suggested oxidative stress.
Conclusion: These findings indicate that LTT induces significant neurochemical alterations, potentially leading to neurotoxicity.
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References
1. Lustig RH. Fructose 2.0: Metabolic, genetic, and societal implications of fructose overconsumption. The Yale J Biol Med .2010; 83(1):3–10.
2. Gomez-Pinilla F. The influences of diet and exercise on mental health through molecular mechanisms. Mol Psych 2008; 13(12):1067–1087.
3. Olney JW. Increase in brain tumor incidence shortly after the introduction of aspartame into the diet. J Neuropathol Exp Neurol 1996; 55(11):1115–1123.
4. Choudhary AK, Lee YY, Bhattacharyya S. A sweet controversy: The effect of artificial sweeteners on the brain. Nutrients 2022; 14(18):3814.
5. Arbind KC, Yeong YL. Neurophysiological symptoms and aspartame: What is the connection? Review. Nutr.Neurosci. 2018; 21(5):306-316. doi:10.1080/1028415X.2017.1288340
6. Emmanuel FT, Gyebi GA, Gyebi GA, Samuel AE, Emeje PI, Ayeni G, Obaje G. Chemical Profiling, In-Silico Investigation and In Vivo Toxicity Assessment of Lacatomtom (A Psycoactive mixture) on selected indices in Albino Wistar Rat.Curr Biol chem 2024;18(4):215-237.
7. Emmanuel FT. Investigation of the Hepatotoxicity of Lacatomtom Drink in Albino Rat. Nig J Biochem Mol Biol 2022;37(1): 26-31.
8. Bewaji CO, Olorunsogo OO, Bababunmi EA. Comparison of the membrane-bound (Ca2+ + Mg2+)-ATPase in erythrocyte ghosts from some mammalian species. Comp Biochem Physiol. 1985; 82B:117-22.
9. Fleschner CR, Naomi K-F, Wibert GJ. Phosphorylated intermediates of two hepatic microsomal ATPases. Biocheml J. 1985; 226(3):839-845.
10. Peter R, Paolo G, Ernesto C. A lipid requirement for the (Ca2+ + Mg2+)-activated ATPase of erythrocyte membranes. Archives of Biochem and Biophy. 1977; 179(2):578-583. https://doi.org/10.1016/0003-9861(77):90146-1
11. Ellman L, Diane CK, Valentino Ajr, Robert MF. A new and rapid colorimetric determination of acetyl cholinesterase activity. J Biocheml Pharmaco 1961;7(2):88-90,IN1,91-95.https://doi.org/10.1016/0006-2952(61):90145-9
12. Jetz NW, Rinker A, Shaw L. IFCC Methods for the Measurements of Catalytic Concentration of Enzymes. Part 5. IFCC Method for Alkaline Phosphatase. J Clin Chem and Clin Biochem 1983; 21:731-748.
13. Bortolato M, Pivac N, Seler DM. The role of monoamine oxidase in neuropsychiatric disorders. Curr Top Med Chem 2010; 10(2): 207–220.
14. Grace AA. Dysregulation of the dopamine system in the pathophysiology of schizophrenia and depression. Nat Rev Neurosci 2016; 17(8):524–532.
15. Berke JD. What does dopamine mean? Nat Neurosci 2018; 21(6): 787–793.
16. Sulzer D, Surmeier DJ. Neuronal vulnerability, pathogenesis, and Parkinson’s disease: insights from studies of dopamine neurons in vivo. Neuron 2013; 80(2): 429–438.
17. Cools R, Roberts AC, Robbins TW. Serotonergic regulation of emotional and behavioural control processes. Trends Cogn Sci 2011; 15(4): 215–227.
18. Baumann MH, Partilla JS, Lehner KR. Psychoactive “bath salts”: Compounds, mechanisms, and toxicity. Mol Neurobiol 2014; 49(2): 574–588.
19. Blusztajn JK, Wurtman RJ. Choline and cholinergic neurons sci. 1983; 221(4611): 614–620.
20. Mesulam MM, Guillozet A, Shaw P. Butyrylcholinesterase involvement in Alzheimer's disease: A comparative study. Annals of Neurol 2002;51(2): 164-172.
21. Hampel H, Mesulam MM, Cuello AC. The cholinergic system in the pathophysiology and treatment of Alzheimer's disease. Brain 2018;141(7): 1917-1933.
22. Wright CI, Geula C, Mesulam MM. Neuropathologic studies of cholinergic function in aging and Alzheimer's disease. Handbook of Clinical Neurol2013; 118:373-384.
23. Skou JC. Nobel Lecture: The identification of the sodium pump. Biosci Reports 1998; 18(4):155-170.
24. Crivelli SM, Gaifullina A, ChattonJY.Exploring the role of mitochondrial uncoupling protein 4 in brain metabolism, implications for Alsheimer’s disease.Front Neurosci 2024;18:1483708
25. Gibson GE, Blass JP. The role of oxidative stress in neurodegenerative diseases like Alzheimer's disease. J Alzheimer's Disease 2007; 12(2):125-136.
26. Carafoli E. The calcium-signaling saga: Tap water and protein crystals to cellular physiology and disease. FEBS J 2017; 284(4):687-695.
27. Swaminathan R. Magnesium metabolism and its disorders. Clin Biochemist Reviews 2003; 24(2): 47-66.
28. Rahman A, Kruck TP, McLachlan DR. AChE as a potential biomarker for neurodegenerative diseases.J Neurochem 2020; 154(4):377-390.
29. Giacobini E. Cholinesterase inhibitors: New roles and therapeutic alternatives. Pharmaco Res 2017; 117:192-196.
30. Pohanka M. Inhibitors of acetylcholinesterase and their impact on physiology and function of cholinergic system. Neurophysio 2014; 46(2):183-192.
31. Bartus RT, Dean RL, Beer B, Lippa AS. The cholinergic hypothesis of geriatric memory dysfunction. Sci 2018; 217(4558):408-414.
32. Haider S, Saleem S, Perveen T, Tabassum S. Age-related learning and memory deficits: Role of neurotransmitters and effect of polyphenols. Neurosci Res 2016; 118: 48-62.
33. Colović MB, Krstić DZ, Lazarević-Pašti TD, Bondžić AM, Vasić VM. Acetylcholinesterase inhibitors: Pharmacology and toxicology. Curr Neuropharmacol 2013; 11(3):315-335.
34. Pacher P, Beckman JS, Liaudet L. Nitric oxide and peroxynitrite in health and disease. Physiological Reviews2007; 87(1):315-424.
35. Jain S, Maltepe E, Lu M, Simon C, Bradfield CA, Gassmann M. Nitric oxide and oxidative stress in neurodegenerative diseases. Neurosci Res 2011; 118:48-62.
36. Liu Y, Xu Z, Yang G, Li L. Neuroinflammation and oxidative stress in neurodegenerative diseases. Neurochemical Res 2017; 42(4):1130-1141.
37. Olumorin OI, Emmanuel FT, Mohammed N, Emmanuel JO. Effect of Lacatomtom Drink on Spatial Memory in Male Wistar Rat. Trop J Phytomed and Pharmaceu Sci 2025; 4(3):123 – 125.http://www.doi.org/10.26538/tjpps/v4i3.3
38. Akomolafe SF, Akinyemi AJ, Ogunsuyi OB, Oyeleye SI, Oboh G, Adeoyo OO. Effect of Caffeine, Caffeic Acid and Their Various Combinations on Enzymes of Cholinergic, Monoaminergic and Purinergic Systems Critical to Neurodegeneration in Rat Brain-In Vitro. NeuroToxicol 2017; 62:6–13.10.1016/j.neuro.2017.04.008
39. Anderson GH, Catherine NL, Wolever TMS, Manjoo P, Bruce-Thompson LA. Effect of sucralose on hunger and satiety in healthy adults. The Am J Clin Nutr 2016; 104(6): 1461-1468.
40. Emmanuel FT, Gideon AG, Akor ES, Emeje PI, Ayeni G,Pepple NM, Yahaya J, Oka S, Adams O, Gideon O. Chemical profiles and Assessment of Lacatomtom (A Psychoactive Mixture) on Selected Indices in Albino Wistar Rats. Current Chemical Biol2024; 18(4) :215-237.
41. Renwk AG. The fate of dietary phenylalanine and tyrosine and their effects on brain neurotransmitter synthesis and turnover. J Nutr Biochem 1985; 1(3), 169-185.
42. Sharma A, Amarnath S, Thulasimani M, Ramaswamy S. Artificial sweeteners as a sugar substitute: Are they really safe? Ind J Pharmacol, 2016; 48(3): 237–240
43. Smith AB, Jones CD. A sensitive spectrophotometric method for the simultaneous determination of dopamine and serotonin in brain tissue using o-phthalaldehyde derivatization.J Neurosci Met 2015;250:123-130.
44. Schlumpf M, Lichtensteiger W, Langemann H, Waser PG, Hefti FA .fluorimetric micromethod for the simultaneous determination of serotonin, noradrenaline and dopamine in milligram amounts of brain tissue. Biochem. Pharmacol. 1974;23:2337-2346.
45. Gornall AG, Bardawill CJ, David MM.Determination of serum proteins by means of the biuret reaction. J Biol Chem. 1949 ;177(2):751-66.
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